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The goal of the mRNA-seq alternative splicing challenge is to assess the accuracy of the reconstruction of alternatively spliced mRNA transcripts from Illumina short-read mRNA-seq. Reconstructed transcripts will be scored against Pacific Biosciences long-read mRNA-seq. The ensuing analysis of the transcriptomes from mandrill and rhinoceros fibroblasts and their derived induced pluripotent stem cells (iPSC), as well as the transcriptome for human Embrionic Stem Cells (hESC) is an opportunity to discover novel biology as well as investigate species-bias of different methods.